NimaGen has put a raw proficiency on the market , namedReverse Complement PCR . It is a young & novel Amplicon ground place enrichment method acting for Next Generation Sequencing . With this technique , a unsubtle spectrum of nucleotides can be analyse accurately and the risk of crossbreeding contamination or carryover as well as the number of actions to be performed has been significantly reduce , for sure when much material has to be studied . " It has interesting practical software in the medical world , for forensic purposes and for all forms of research . That sure enough also applies to the plant humans " , says researcher Walter van der Vliet .
Success in fostering largely depends on the stop number at which one can select . Therefore , one increasingly often makes usance of specialized research institutions and companies , which can look into the genome with raw in advance techniques and crack the genetic computer code . This is cry Sequencing ; determine the molecular order of the DNA , and by marking and selecting specific components , class of ( intuitive ) crossings can thus be shorten .
Double improvementThere are various techniques on the market with which this is possible . For most of these , DNA must first be replicated twice to be able-bodied to analyze it properly . The samples must then be added together , on which the actual analytic thinking can take place . “ It works , but has two disadvantage . Cross - contamination can occur in the different footstep , which can have a very negative effect on issue . In addition , it is labor - intensive , because many steps must be followed and many sample must be analyzed separately . With our technology , this mental process is easy to automatize , both step are combined and more outcome can be distil from more sample at the same clip . ”
The technique originates from the infirmary and was evolve by two researchers from the National Health Service in a laboratory in Salisbury , United Kingdom . Research was carried out into the genetical sensitivity for white meat Crab , cystic fibrosis and all form of other disease . NimaGen has exclusively certify the patent . The physical Cartesian product does not comprise of any machine but of boxes containing the required reagents .
NimaGen has now developed a number of covering for RC - PCR . For example , they are work on the so - called liquid biopsy covering , with which ancestry samples can be check for the front of DNA from malignant neoplastic disease cells . A second program - no doubt others will follow - has been find in making tests for company in the plant diligence . For industrial plant reproduction , these kit are commercialize under the name " GreenoType " . These are customs made , or specifically made for customers with a certain research question . The stock breeder must bring in knowledge , so to speak , on which markers should be added and what type of property should be searched for .
" There are two projects in a last stage ; one client has many DNA markers and just wants to do marking - assisted breeding ; the other has just a very small set of markers on the basis of which he wants to see if his product and the ejaculate are of sufficient quality . Because our test is so sore , it means that rather of finding 1 out of the 100 regretful seeds , he will before long be capable to receive 1 out of the 1000 unsound seeds . That can prevent a substantial personnel casualty of income . "
Faster , more , and more accurateThanks to this technique , it is possible to map a larger part of the genome faster and more accurately . giving players , of line , bet on a inherited approach to upbringing , but when they start try out , they often do it again the old direction , Walter explains . “ We are at the cutting edge of what is possible . For the clock time being the engineering is not cheap , but as many more properties can be canvas at once , it is credibly comparatively tinny again . If the customer sees that he can make a decision on much a larger basis , so that all of a sudden he has 50 characteristics in focus instead of just those 5 that he had in the past tense , then he will certainly be concerned . We see that for exemplar at the PAG Conference in San Diego ( USA ) last January and at Seed Meets Technology in Zwaagdijk ( Netherlands ) . ”
Outsmarting the competitionNow it is important to build more references . The possibilities are dateless . bacterium can be detected in blood or tissue paper , but also in plant cloth or in the soil . All kinds of examination can be developed for specific mintage or it can be used , for instance , to see whether or not an early cross or transmissible manipulation via CRISPR - Cas has succeeded . “ However , we depend on our customer for concrete results . We can show that it works , we do test on reference textile and send it back , but they will put it back to their gold standard . To what extent they attain ( private-enterprise ) vantage from it - we think they will , but we will indeed have to hear that from them . ”
NimaGen originated in 2011 from Biolegio , a ship’s company that makes oligonucleotides ( oligos ) or small piece of DNA . These attend as raw material for various product that are marketed by NimaGen . Eight people are now work there , but it is require that in the coming year it will take on three or four the great unwashed to actually be able-bodied to launch the projects on the shelf .
For more information : NimaGen B.V.P.O. Box 916500 AB NijmegenT : +31(0)24 820 02 41E:[email protected]www.nimagen.com
© FloralDaily.com/Geert Peeters
